Tumor Necrosis Factor Alpha Gene Level in Patients with Tympanosclerosis
Yasser M Mandour, Mohammed Shendy, Ahmed H El-Zobeir, Ayman A Mohammady, Shuzan A Mohammed, Naslshah G Kazem
Chronic otitis media with effusion, COME, OM, Otitis media, PCR, Polymerase chain reaction, QRT-PCR, Quantitative real time PCR, TNF-α, Tumor necrosis factor alpha
Citation Information :
Mandour YM, Shendy M, El-Zobeir AH, Mohammady AA, Mohammed SA, Kazem NG. Tumor Necrosis Factor Alpha Gene Level in Patients with Tympanosclerosis. Int J Otorhinolaryngol Clin 2023; 15 (1):1-4.
Background: Tympanosclerosis is the calcification of middle ear connective tissue, including tympanic membrane, which sometimes occurs after middle ear inflammation. Tumor necrosis factor (TNF)-α tends to induce and maintain inflammatory responses in various diseases, including chronic otitis media with effusion (COME).
Aim: To assay plasma level of TNF-α gene expression in patients with tympanosclerosis due to ventilation tube insertion.
Methods: Sixty patients were included in this study. Patients were divided into two groups: Group A: 60 patients, according to inclusion and exclusion criteria, and Group B: 20 volunteers. Patients were subjected to history taking, full clinical examination, and laboratory investigation of TNF-α gene expression [quantitative real-time PCR (qRT-PCR)].
Results: There was a statistical difference between groups according to TNF-α gene expression, as the mean TNF-α in the case group (3.87) was statistically higher than in the control group I, p < 0.001. There was a statistical difference in TNF-α level regarding the number of tube insertion (p = 0.005) and duration of secretory otitis media (OM) (p = 0.042). However, there was no statistical difference in TNF-α level regarding medication use or episodes of acute OM.
Conclusion: The degree of gene profile expression for TNF-α in selected middle ear structures with tympanosclerosis was statistically important, higher than the control group.
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